WebJul 23, 2024 · Whole-cell patch-clamp analysis revealed that SA activation of the S-type anion channel is impaired in cpk3-2 cpk6-1guard cell protoplasts (GCPs) but not in ost1 … WebAbstract: In recent years a slow-type anion channel (SLAC) family composing five members, including slow anion channel associated 1 (SLAC1) and four SLAC1 homologs (SLAHs), has been identified in Arabidopsis thaliana. The anion channels are activated by calcium-dependent protein kinases (CPKs) and play crucial roles in mediating anion fluxes ...
Guard Cell Expression of CPK3 and CPK6 CDPKs (A
Webrescence between SLAC1 and CPK6 and 21 was weaker (Fig. S2A). BiFC-signals between SLAC1 and CPK3 were even fainter (Fig. S2A). To exclude the possibility that a high expression of two proteins in Xenopus oocytes leads to interactions already, we tested SLAC1 together with CPK31, a close homolog of CPK21 and 23 within the CDPK kinase … WebApr 27, 2010 · To study SLAC1 activation via the calcium-dependent ABA pathway, we studied the SLAC1 response to CPKs in the Xenopus laevis oocyte system. Split YFP … link pharmacy east end road
Reconstitution of abscisic acid activation of SLAC1 …
WebJan 1, 2013 · The cpk3 cpk6 double mutant is impaired in ABA-activation of slow-type anionic channels and Ca 2+ permeable channels, resulting in decreased ABA-induced stomatal closure [80]. Despite a weak in vivo interaction with the major guard cell Cl – channel SLAC1 [22] , AtCPK6 but not AtCPK3 was recently shown to phosphorylate … WebSep 9, 2014 · The kinases that activate SLAC1 are CIPK11/CBL5, CIPK23/CBL1or CBL9, and CPK3, 6, 21, 23 [74, 111,116,140,200]. Although these kinases activate SLAC1 in Xenopus oocytes, they phosphorylate ... WebDec 4, 2024 · These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity. Remodelling of the actin cytoskeleton occurs during ... link pharmacy head office