2024 Raf1 and rbcx

Raf1 and rbcx

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August undefined, 2024

WebSep 1, 2024 · Form I Rubisco has emerged as a paradigm of assisted assembly involving multiple auxiliary factors, including RbcX, Rubisco accumulation factors (Raf)1 and Raf2, and bundle sheath defective-2 (BSD2) [ 31 ]. WebRAF1, RBCX and BSD2 in assisting the dimerization of RbcL and subsequent stabilisation of the L 8 core before the formation of the final holoenzyme with RbcSs24-27. In a recent breakthrough, Aigner and co-workers were able to produce functional Arabidopsis Rubisco in E. coli by co-expressing five additional chaperones: RBCX, RAF1, RAF2, BSD2 ...

Chaperone Machineries of Rubisco – The Most Abundant Enzyme

WebMar 13, 2024 · Expression of Arabidopsis Rubisco in E. coli provided some insight regarding the potential roles for Raf1 and RbcX in facilitating RbcL oligomerization, for Bsd2 in … WebSep 14, 2024 · The four pCDF vectors contain tobacco rbcX and RAF1 genes as well as RAF2 and BSD2 genes from either Arabidopsis or tobacco under T7 promoters (Fig. 1b ). The first pCDF vector has the tobacco... porch shirt

MsTHI1 overexpression improves drought tolerance in transgenic …

WebOct 3, 2014 · We considered the possibility that RAF1, RAF2 and/or BSD2 might mimic RbcX function in chloroplasts, but we were unable to obtain direct evidence of the composition and order of formation of these putative intermediates. WebAug 31, 2012 · Rubisco Subunit Genes Are Normally Transcribed and Translated in raf1 Mutants To ascertain whether RAF1 is required for the accumulation of rbcL or RBCS transcripts, gel blot analysis was performed on total RNA from wild-type, raf1, and bsd2 seedling leaves ( Figure 3A ). WebMar 21, 2024 · RAF1 (Raf-1 Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with RAF1 include Leopard Syndrome 2 and Noonan … sharp a066tb

Rubisco Assembly in the Chloroplast - Semantic Scholar

Rubisco Assembly in the Chloroplast - PMC

WebPrevious studies showed that RbcX (6) and RAF1 (7) both bind and stabilize pairs of RuBisCO large subunits during the multistep assembly process, suggesting that they might WebMay 13, 2024 · 7XSD PubMed Abstract: RuBisCO is the most abundant enzyme in nature, catalyzing the fixation of CO 2 in photosynthesis. Its common form consists of eight RbcL and eight RbcS subunits, the assembly of which requires a series of chaperones that include RbcX and RuBisCO accumulation factor 1 (Raf1) ... Macromolecules sharp a1 robotWebMentioning: 14 - The most prevalent form of the Rubisco enzyme is a complex of eight catalytic large subunits (RbcL) and eight regulatory small subunits (RbcS). Rubisco biogenesis depends on the assistance by specific molecular chaperones. The assembly chaperone RbcX stabilizes the RbcL subunits after folding by chaperonin and mediates … sharp a011sd

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Raf1 and rbcx

RCSB PDB - 7XSD: Cryo-EM structure of RuBisCO assembly …

WebJul 7, 2024 · Although differing in protein structure, both Raf1 and RbcX play roles in Rubisco assembly by promoting RbcL dimerization . In Syn7942, RbcX is not essential for cell … WebJun 12, 2024 · Folded RbcL requires a number of additional Rubisco specific assembly chaperones, including RbcX, Rubisco accumulation factors 1 (Raf1) and 2 (Raf2), and the Bundle sheath defective-2 (BSD2), to mediate the assembly of the RbcL 8 intermediate complex. Incorporation of the RbcS and displacement of the assembly factors generates …

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WebNov 2, 2024 · In most cyanobacteria, RbcX is the product of the rbcX gene that is commonly located in the same operon between the rbcL and rbcS genes, indicating its structural or functional relationship with Rubisco ( Liu et al., 2010; Bracher et al., 2024; Hayer-Hartl, 2024 ).

WebAug 3, 2015 · Raf1 fulfills a role similar to that of the assembly chaperone RbcX, thus suggesting that functionally redundant factors ensure efficient Rubisco biogenesis. Main … WebJan 20, 2024 · An entire set of auxiliary factors, including chaperonin GroEL/GroES and assembly chaperones RbcX or Rubisco accumulation factor 1 (RAF1), is involved in the folding and subsequent assembly of Rubisco subunits. Recently, it has been shown that cyanobacterial RAF1 acts during the formation of the large Rubisco subunit (RbcL) dimer.

WebAug 3, 2015 · Here we analyzed the role of Rubisco accumulation factor1 (Raf1), a dimer of ∼40-kDa subunits. We find that Raf1 from Synechococcus elongatus acts downstream of chaperonin-assisted RbcL folding by stabilizing RbcL antiparallel dimers for assembly into RbcL8 complexes with four Raf1 dimers bound. WebFeb 23, 2024 · The Δ raf1 was generated, as described in our previous work [ 17 ]; Δ sll1384 was a kind gift from Professor Dirk Schneider. Protein purification E.coli pellets were suspended in a 50-mM phosphate buffer (pH 6.5 for DnaJs and pH 7.5 for DnaK), with 150-mM NaCl, 1-mM PMSF, and 3-mM β-ME.

WebRaf1 Is Important for Cyanobacterial Physiology. To study the roles of Raf1 in cyanobacteria, a raf1 deletion Syn7942 mutant (Δraf1) was generated (SI Appendix,Fig.S1A and …

WebDec 8, 2024 · Aigner et al. clarify the role of all four of the known plant RuBisCO-specific chaperones: RbcX, RAF1, RAF2, and BSD2. Previous studies showed that RbcX ( 6) and RAF1 ( 7) both bind and stabilize pairs of RuBisCO large subunits during the multistep assembly process, suggesting that they might be functionally redundant. porch shiplapWebJul 7, 2024 · Reconstruction and cryo-electron microscopy (cryo-EM) of the Syn7942 RbcL-Raf1 complex demonstrate that Raf1 is critical for assembly of Rubisco holoenzymes by mediating the formation of RbcL dimer and RbcL dimer–dimer interactions. sharp a024sdWebRAF1 is listed in the World's largest and most authoritative dictionary database of abbreviations and acronyms RAF1 - What does RAF1 stand for? The Free Dictionary sharp 9 musicWebJul 12, 2014 · Co-expression of rbcL, rbcS and raf1 genes led to the formation of fully assembled and active Rubisco with a yield comparable to the variant where Rubisco structural genes are co-expressed with rbcX (Fig. 3C). When raf1 was co-expressed with rbcL alone, formation of a novel RbcL–RAF1 assembly intermediate was observed (Fig. 3B). sharp a202WebFeb 3, 2024 · RAF1 (RuBisCO Accumulation Factor 1), found in eukaryotes and certain cyanobacteria, operates similarly to RbcX in that it also stabilizes the LS dimer before SS incorporation (Feiz et al., 2012). Folded LS is released from the GroEL complex in the presence of RAF1, and LS dimers have been found to interact with RAF1 dimers (Bracher … porch shopWebRaf1–RbcX interface are relatively variable among Raf1 and RbcX homologs (Supplementary Fig. S3). It indicates that Raf1 and RbcX do not possess speciﬁc … porch shoe storage ideasWebSep 8, 2024 · RbcX2, chaperonin-like RbcX protein 2. Cu/Zn-SOD, superoxide dismutase [Cu-Zn]. ... Transgenic overexpression of rubisco subunits and the assembly factor RAF1 are beneficial to recovery from drought stress in maize. Environ. Exp. Botany 177, 104126. 10.1016/j.envexpbot.2024.104126 ... sharp a207 manual