Size ha and myc
WebbMYC (bHLHe39, c-Myc, MYCC) protein expression summary. Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' 1, 2.Activates the transcription of growth-related genes 3, 4.Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis … WebbThe Myc-tag allows simple and efficient affinity purification of the tagged protein using Myc-tag specific antibody conjugated to agarose-beads. The Myc-tag (EQKLISEEDL-tag) also can be used for detection in western blot by using a Myc-tag-specific antibody. Myc-tag can be added to either C- or N-terminus of a protein for expression in ...
Size ha and myc
Did you know?
Webb21 mars 2024 · Complete information for MYC gene (Protein Coding), MYC Proto-Oncogene, BHLH Transcription Factor, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium. ... Size: 454 amino acids Molecular mass: 50565 Da Protein existence level: PE1 Quaternary structure: WebbSize of the Myc-tag Number of amino acids: 10 Molecular weight (MW): 1203.31Da 7. Specifications of the Myc-tag Theoretical isoelectric point (pI): 4.00 Total number of …
Webb23 feb. 2024 · Myc regulates a large number of protein-coding or non-coding genes that are involved in distinct cellular functions, including cell cycle, protein biogenesis, cell adhesion, metabolism, signal... The first use of epitope tagging was described by Munro and Pelham in 1984. The FLAG-tag was the second example of a fully functional, improved epitope tag, published in the scientific literature. and was the only epitope tag to be patented. It has since become one of the most commonly used protein tags in laboratories worldwide. Unlike some other tags (e.g. myc, HA), where a monoclonal antibody was first isolated against an existing protein, then the epitope wa…
Webb12 apr. 2024 · As both c-Myc and c-Jun are notably downstream targets of the canonical and non-canonical Wnt signaling pathways, respectively, which are both down-regulated by Sfrp1, as well as two oncogenes involved in breast cancer progression, we questioned if their expression could also be modulated by parity status, E2, or HA ex vivo, as Sfrp1 … Webb14 apr. 2024 · Western blots were probed by α HA or α Myc antibodies, respectively. Signals revealed an expected SMXL5-3xHA protein size of ~120 kDa. The size of the detected 6xMyc-OBE3 protein (~100 kDa ...
Webb20 okt. 2024 · HA tag is one of most commonly used tag systems. Other commonly used ones include c-Myc and His6. Schüchner S et al suggested that clone 12CA5, raised against a 36-residue peptide from hemagglutinin, might display context-dependent affinities, based on their results with myc tag antibodies [ 4 ].
WebbDescription. c-Myc tag peptide is a synthetic peptide with a molecular weight of 1,203 Da. The peptide sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. A complete kit containing this peptide exists for the isolation of c-Myc-tagged protein (code # 3305).This kit contains c-Myc tag beads that can be … scheels running shoes for menWebbUse prepacked HIC chromatography columns for protein capture, intermediate purification, or polishing steps. Use multimodal or mixed mode chromatography for increased selectivity, high salt conditions, or process efficiency. Use size exclusion columns to separate proteins and other biomolecules based on size and shape. rustic wedding shower centerpiecesWebbHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does ... scheels return policy daysWebb11 aug. 2024 · Fusion tags relevant for this manuscript can vary greatly in size, from large tags such as the Aequorea victoria green fluorescent protein (GFP) with a size of 26,9 kDa, to small peptide tags including multiple copies of c-myc and HA, each around 1 kDa. rustic wedding rsvp cardsWebb3 juni 2024 · It’s also known as MRTL, MYCC, and bHLHe39. MYC genes are classified as protein-coding genes. This means they are genes that translate the genetic code in your body that specifies the correspondence between codons and amino acids. MYC genes specifically control various aspects of cell growth in your body and regulate your cellular … scheels scopes for saleWebbThe hemagglutinin (HA) tag is a 9-amino acid long peptide corresponding to residues 98-106 of the human influenza HA molecule, an ~63kDa surface glycoprotein required for … scheels return policy on firearmsWebb1 juni 2002 · In this study, we have used Omomyc as a probe to interfere with two distinct functions of Myc: ( a) cytoskeletal reorganization; and ( b) apoptosis, which can be involved in the development or maintenance of tumors (9). MATERIALS AND METHODS Expression Vectors and Cell Lines. rustic wedding supplies cheap