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Thy1-gfp mice

Webb19 apr. 2024 · 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J These thy1-YFP-16 transgenic mice express yellow fluorescent protein at high levels in motor and sensory neurons, as well as in subsets of central neurons. This line provides a strong and specific vital marker for … Webb29 juli 2014 · GFP + Vit.A + and GFP + Vit.A − fractions were sort purified from Col-GFP mice (n = 6) after BDL (20 d). Expression of myofibroblast marker (α-SMA), HSC markers (desmin, GFAP, CD146), and PF markers (elastin, Thy1) were analyzed by immunocytochemistry using specific antibodies or isotype matched controls (40× …

(PDF) Tissue clearing of both hard and soft tissue organs with the ...

http://www.nrcmm.cn/article/view-64.html WebbTransmission electron microscopy revealed FLAG immunogold within mitochondria. Compared with Thy1.2-positive retinal ganglion cells (RGCs), quantification was 38% for FLAG-positive RGCs and 65% for GFP-positive RGCs. Thy1.2 positive-RGC counts in AAV-ND4FLAG were similar to counts in control eyes injected with AAV-GFP. fitcon sugarland https://delasnueces.com

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WebbMice of the thy1GFP-M line have been engineered for selective expression of green fluorescent protein (GFP) in neuronal populations. Here, we report that TPF microscopy reveals, at the brain surface of these mice, also motile non-neuronal GFP+ cells. Webb7 jan. 2024 · In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1 -GCaMP6s transgenic mice using 2-photon (2P) microscopy. Webb12 mars 2024 · Beyond mice, recently developed transgenic Thy1-GFP rat reporters have allowed for the study of nerve injury in a larger and longer-living animal model. For example, facial nerve re-innervation of the zygomaticus muscle was assessed 4 weeks after crush injury via confocal microscopic imaging of Thy1GFP labeled neurons in rats [46, 47]. fitcon tickets

IL-4 expressing cells are recruited to nerve after injury and …

Category:Mapping of fluorescent protein-expressing neurons and axon

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Thy1-gfp mice

Thy1 marks a distinct population of slow-cycling stem cells in the ...

Webb1 juli 2024 · These mice are useful for examining nerve formation, injury, repair, and degeneration. • Marker proteins Thy1 and Nestin are used to visualize different subsets of neurons. Abstract Recombinant DNA technologies have enabled the development of … WebbThy1-GFP− cells will not be traceable due to the allogeneic nature of the transplant. (C) ... Beyond the initial follow-up of 2 weeks, mice injected with Thy1-GFP+ RGCs as P2–P4 pups retained donor cells beyond 12 months post-transplantation, ...

Thy1-gfp mice

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Webb29 maj 2024 · Adult (60 days of age) Thy1-EGFP mouse brain was cleared following the PEGASOS passive immersion procedure. The dorsal-to-ventral thickness of the brain is around 4 mm after processing. Webb1 okt. 2000 · Neuromuscular junctions from thy1-YFP line H (a), thy1-GFP line H (b), thy1-CFP line D (c), and thy1-RFP line 8 (d) transgenic mice. In each case, muscles were labeled with bungarotoxin, to confirm perfect apposition of pre- and postsynaptic specializations …

Webb23 juli 2010 · J.R. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51). Here, we provide a comprehensive mapping of labeled cell types throughout the central nervous system in adult and postnatal (P0-P30) … Webb16 apr. 2008 · (f) Quantification of GFP fluorescence intensity at the soma normalized to intensity of GFP-labeled pyramidal soma in Thy1-GFP mice; n = 15 cells for each group. The intensity range for both Thy1-GFP and AAV-labeled somata was large, only the brightest cells were selected for this particular comparison (see Methods for details).

Webb13 apr. 2024 · Thymi were isolated from 4-week-old CD45.2 + Eomes-GFP + mice and CD4 + thymocytes cells were depleted by incubating thymi with anti-mouse CD4 antibody conjugated to Biotin (clone RM4–5 ... WebbCD90 (Thy1) Antibodies Antibodies that detect CD90 (Thy1) can be used in several scientific applications, including Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry and Functional assay. These antibodies target CD90 (Thy1) in Human, Mouse, Rat, Porcine and Rabbit samples.

Webb9 juli 2024 · The adult Thy1-GFP mice aged eight months (n = 4 male and n = 12 female) used for these experiment were kindly provided by Dr. B. Brunne and Prof. M. Frotscher (Center of Molecular Neuroscience ...

Webb7 aug. 2013 · Thy1-GFP-M is a transgenic reporter line in which eGFP is expressed in a subset of layer II/III and V cortical neurons ( Feng et al., 2000 ). The R6/2 mice represent a well characterized model of HD. fit content to screenWebb011070 B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ These thy1 -GFP-S transgenic mice show EGFP labeling in the superficial layers of the neocortex, and may be useful in neurobiological studies for fluorescent labeling of a variety of cerebral cortex interneuron subsets. fit content to screen on computerWebb17 aug. 2024 · Thy1-GFP/ Syngap1+/+ or Thy1-GFP/ Syngap1+/− mice were implanted with a chronic cranial window and recovered for at least 2 wk before IOS imaging. IOS was used to identify the cortical location activated by the deflection of the β whisker. fit content to frame indesign shortcutWebb19 apr. 2024 · (E) High-magnification confocal image of Axl + microglial processes contacting dendritic spines (arrow) in a 5XFAD;Thy1-eGFP mouse at 5 months. Scale bars, 2 μm. Quantification of relative frequency of observed contacts between control (n = 42 dendrites >10 μm long from n = 8 mice) and 5XFAD mice (n = 44 dendrites >10 μm long … can hackers access your iphoneWebb6 apr. 2024 · We had previously shown that THY1 (CD90) is a tumor suppressor in nasopharyngeal carcinoma (NPC) and that its down-regulation and loss of expression are associated with tumor metastasis, yet the mechanism leading to such effects remains unknown. In this study we show that tumor invasion could be suppressed by THY1 via … can hackers access your phoneWebbFurthermore, the role of BDNF for stimulation-induced changes in spine density was assessed in heterozygous Thy1-GFP x BDNF+/- mice. Results: Combined DCS/eFS rapidly increased spine density during stimulation and changes outlasted the intervention for 24 h. fit continuing edWebbA biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields fitco nuffield